2WW1
Structure of the Family GH92 Inverting Mannosidase BT3990 from Bacteroides thetaiotaomicron VPI-5482 in complex with Thiomannobioside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.240, 153.110, 185.210 |
| Unit cell angles | 90.00, 91.37, 90.00 |
Refinement procedure
| Resolution | 185.160 - 2.250 |
| R-factor | 0.18904 |
| Rwork | 0.188 |
| R-free | 0.21817 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.209 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.140 | 0.470 |
| Number of reflections | 174544 | |
| <I/σ(I)> | 7.1 | 3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% 3350 PEG, 0.1 M (4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID) (PH 7.0), 0.15 M K2SO4 |
