2WVZ
Structure of the Family GH92 Inverting Mannosidase BT3990 from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.900, 150.190, 382.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 191.140 - 2.400 |
| R-factor | 0.20394 |
| Rwork | 0.201 |
| R-free | 0.25718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.321 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.490 |
| Number of reflections | 156201 | |
| <I/σ(I)> | 6.2 | 2.1 |
| Completeness [%] | 88.4 | 89.3 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% V/V 3350, 100MM MIBS BUFFER (PH 6.0) |
