2WVP
Synthetically modified OmpG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.560, 69.810, 59.130 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f1c |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.710 |
| Number of reflections | 15345 | |
| <I/σ(I)> | 16.7 | 2.2 |
| Completeness [%] | 98.9 | 95.4 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.75 | 50 MM CACODYLATE, PH 5.75, 1.2 M SODIUM FORMATE AND 28% PEG400 |
