2WVM
H309A mutant of Mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27 in complex with GDP-alpha-D-Mannose and Mg(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.766, 112.766, 199.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 98.190 - 2.977 |
| R-factor | 0.17643 |
| Rwork | 0.174 |
| R-free | 0.23163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.642 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.160 |
| High resolution limit [Å] | 2.977 | 2.977 |
| Rmerge | 0.040 | 0.370 |
| Number of reflections | 25598 | |
| <I/σ(I)> | 15.4 | 2.09 |
| Completeness [%] | 94.0 | 80.6 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M MAGNESIUM ACETATE, 0.1 M SODIUM CACODYLATE PH 6.5, 30-35% MPD WITH 600 MM ZNCL2 AS AN ADDITIVE, 1-5 MM GDP-ALPHA-D-MANNOSE |
