2WVK
Mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27 apoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.456, 113.456, 195.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.910 - 2.970 |
| R-factor | 0.20122 |
| Rwork | 0.198 |
| R-free | 0.26636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 3.100 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.110 | 1.270 |
| Number of reflections | 26864 | |
| <I/σ(I)> | 19.5 | 2.2 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 18.9 | 18.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M MAGNESIUM ACETATE, 0.1 M SODIUM CACODYLATE PH 6.5, 30-35% MPD WITH 600 MM ZNCL2 |
