2WUR
Atomic resolution structure of GFP measured on a rotating anode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-20 |
| Detector | BRUKER SMART 6000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.987, 59.050, 67.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 0.900 |
| R-free | 0.17400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.034 |
| RMSD bond angle | 0.052 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SHELXD (SHELXE) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 1.000 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.080 | 0.490 |
| Number of reflections | 155426 | |
| <I/σ(I)> | 20.35 | 3 |
| Completeness [%] | 91.4 | 90.3 |
| Redundancy | 19.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP VAPOR DIFFUSION: 2 UL PROTEIN (10 MG/ML IN 20 MM TRIS, PH 8.0) PLUS 2 UL RESERVOIR (40% ETHANOL, 10 % DIOXANE) |
