2WUQ

Crystal structure of BlaB protein from Streptomyces cacaoi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	54.140, 94.580, 61.380
Unit cell angles	90.00, 99.54, 90.00

Refinement procedure

Resolution	47.300 - 1.800
R-factor	0.1626
Rwork	0.161
R-free	0.20099
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dy6
RMSD bond length	0.013
RMSD bond angle	1.423
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.120	0.780
Number of reflections	56421
<I/σ(I)>	10.4	2.6
Completeness [%]	100.0	99.8
Redundancy	5.9	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1