2WUH

Crystal structure of the DDR2 discoidin domain bound to a triple- helical collagen peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-16
Spacegroup name	P 1
Unit cell lengths	33.397, 40.863, 48.917
Unit cell angles	66.19, 88.90, 76.92

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.2019
Rwork	0.202
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2QQJ AND 2V53
RMSD bond length	0.005
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.050	0.280
Number of reflections	27359
<I/σ(I)>	16.8	4.3
Completeness [%]	90.2	67.4
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.0