2WTH
Low resolution 3D structure of C.elegans globin-like protein (GLB-1): P3121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.681, 77.681, 145.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.280 - 2.800 |
| R-factor | 0.27139 |
| Rwork | 0.269 |
| R-free | 0.31948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wtg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.996 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.300 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.060 |
| Number of reflections | 12777 |
| <I/σ(I)> | 23.3 |
| Completeness [%] | 98.4 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.0 M AMMONIUM SULPHATE, 10% GLYCEROL |
