2WT4
Room temperature crystal structure of Helicobacter pylori L- asparaginase at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2009-08-28 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.909, 96.369, 101.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.010 - 1.800 |
| R-factor | 0.15321 |
| Rwork | 0.152 |
| R-free | 0.18477 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.321 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 29828 | |
| <I/σ(I)> | 24.8 | 3.5 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 11.1 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 17.5% (W/V) PEG 4000, 0.1 M MG FORMATE, 0.1 M HEPES-NAOH, PH 7.0 |
