2WT2
Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with tri(N-acetyl-lactosamine)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-03 |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.130, 38.260, 86.520 |
| Unit cell angles | 90.00, 92.12, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.500 |
| R-factor | 0.20365 |
| Rwork | 0.200 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wsu CHAIN A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.274 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 20441 | |
| <I/σ(I)> | 8.8 | 3.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10 MM TRIS-HCL, 1 MM EDTA, 28% (W/V) POLY-ETHYLENE GLYCOL 3350, 300 MM LITHIUM NITRATE, 5 MM DITHIOTHREITOL, 5 MM TRI(N-ACETYL-LACTOSAMINE), PH 8.0 |
