2WSY

CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-11
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	185.100, 61.200, 67.600
Unit cell angles	90.00, 94.70, 90.00

Refinement procedure

Resolution	10.000 - 3.050
R-factor	0.197
Rwork	0.197
R-free	0.29900
Structure solution method	ISOMORPHOUS WITH PDB ENTRY 1WSY.
Starting model (for MR)	1WSY
RMSD bond length	0.003
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	3.050	3.050
Rmerge	0.034	0.210
Total number of observations	28423 *
Number of reflections	13162
<I/σ(I)>	20.2	4.2
Completeness [%]	90.9	89.8
Redundancy	2.1	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		drop consists of equal volume of enzyme and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	bicine	50 (mM)
3	1	drop	pyridoxal phosphate	0.02 (M)
4	1	reservoir	bicine	50 (mM)
5	1	reservoir	spermine	1-2 (mM)
6	1	reservoir	PEG8000	10 (%)