2WSU
Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.691, 77.325, 94.111 |
| Unit cell angles | 90.00, 101.45, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.900 |
| R-factor | 0.20772 |
| Rwork | 0.206 |
| R-free | 0.25915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a3k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.317 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 89027 | |
| <I/σ(I)> | 10 | 3.7 |
| Completeness [%] | 96.1 | 90.1 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10 MM TRIS-HCL PH 8.0, 1 MM EDTA, 25% (W/V) POLY-ETHYLENE GLYCOL 3350, 500 MM SODIUM NITRATE, 2 MM SIALIC ACID |
