2WSK

Crystal structure of Glycogen Debranching Enzyme GlgX from Escherichia coli K-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-03-16
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	76.852, 91.937, 97.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.960 - 2.250
R-factor	0.209
Rwork	0.209
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vr5
RMSD bond length	0.006
RMSD bond angle	1.400
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.150	0.240
Number of reflections	30337
<I/σ(I)>	6.5	3.4
Completeness [%]	90.8	96.9
Redundancy	12.3	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		MPD 47%, NA-CITRATE 0.2M, PEG 3350 4%, HEPES PH8.0, pH 8.5