2WSI
Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.871, 36.861, 79.231 |
| Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
| Resolution | 39.582 - 1.900 |
| R-factor | 0.1768 |
| Rwork | 0.174 |
| R-free | 0.23120 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 22638 | |
| <I/σ(I)> | 18.3 | 4.3 |
| Completeness [%] | 96.5 | 96.5 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17 TO 25% ETHYLENE GLYCOL, 50-100MM AMMONIUM SULPHATE |
