2WRZ
Crystal structure of an arabinose binding protein with designed serotonin binding site in open, ligand-free state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.760, 86.300, 116.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.960 - 2.200 |
| R-factor | 0.21301 |
| Rwork | 0.210 |
| R-free | 0.27159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5abp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.248 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.170 | 0.870 |
| Number of reflections | 41190 | |
| <I/σ(I)> | 14.8 | 2.33 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 7.27 | 7.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20% PEG 3350, 0.2M (NH4)CITRATE, 2UMOL/ML SEROTONIN, pH 8.0 |
