2WRT
The 2.4 Angstrom structure of the Fasciola hepatica mu class GST, GST26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.310, 92.501, 166.422 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 166.670 - 2.400 |
| R-factor | 0.20926 |
| Rwork | 0.205 |
| R-free | 0.28102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fhe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 109322 | |
| <I/σ(I)> | 7 | 1.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MDL1 CONDITION NUMBER 18 (0.2 M NA ACETATE, 0.1 M NA CACODYALTE, PH 6.5, 30% W/V PEG 8000) |
