2WR6
Structure of the complex of RBP4 with linoleic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 155 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.558, 102.558, 73.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.520 - 1.800 |
| R-factor | 0.18887 |
| Rwork | 0.187 |
| R-free | 0.22676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qab |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.305 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.070 | 1.020 |
| Number of reflections | 26657 | |
| <I/σ(I)> | 12.21 | 1.29 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5-4.2M NACL, 0.1M HEPES, PH 7.2 |
