2WQY

Remodelling of carboxin binding to the Q-site of avian respiratory complex II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 1 21 1
Unit cell lengths	118.700, 200.753, 67.631
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	64.090 - 2.100
R-factor	0.18585
Rwork	0.184
R-free	0.22283
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0066)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	50 G/L PEG-3350, 25 ML/L ISOPROPANOL, 15 ML/L PEG-400 0.05 M NA-HEPES, 0.01 M TRIS-HCL, 0.0005 M MNCL2, 0.0013 M MGCL2, 0.0015 M NA-AZIDE, 0.00025 M NA-EDTA, CARBOXIN, PH 7.50, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K