2WQX
InlB321_4R: S199R, D200R, G206R, A227R, C242A mutant of the Listeria monocytogenes InlB internalin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | RIGAKU SATURN 944PLUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.700, 57.450, 64.300 |
| Unit cell angles | 102.99, 90.21, 93.56 |
Refinement procedure
| Resolution | 38.630 - 2.030 |
| R-factor | 0.19729 |
| Rwork | 0.194 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6t |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.799 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.120 | 0.410 |
| Number of reflections | 33525 | |
| <I/σ(I)> | 12.65 | 4.02 |
| Completeness [%] | 96.8 | 68.5 |
| Redundancy | 5.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | SITTING DROP VAPOUR DIFFUSION AT 293K WITH 1 UL PROTEIN (3.8 MG/ML IN 50 MM HEPES, PH 7.0, 100 MM NACL) PLUS 0.5 UL RESERVOIR (17% PEG1000MME, 90 MM NA-CITRATE). |
