2WQQ
Crystallographic analysis of monomeric CstII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-20 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 116.634, 116.634, 45.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.250 |
| R-factor | 0.17868 |
| Rwork | 0.177 |
| R-free | 0.21863 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ro7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.366 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 14714 | |
| <I/σ(I)> | 18.4 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M AMMONIUM CITRATE TRIBASIC, 10% PEG 3350, PH 8.0 |
