2WQI

Crystal structure of the human p73 tetramerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	H 3
Unit cell lengths	76.110, 76.110, 79.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.780 - 1.700
R-factor	0.211
Rwork	0.209
R-free	0.25400
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.005
RMSD bond angle	0.981
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.800	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.240
Number of reflections	18950
<I/σ(I)>	17.9	5
Completeness [%]	100.0	100
Redundancy	6.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	290	SITTING DROP VAPOR DIFFUSION AT 17 DEGREE C. PROTEIN SOLUTION: 15 MG/ML IN 20 MM TRIS (PH 8.5), 50 MM NACL. CRYSTALLIZATION BUFFER: 0.1 M SODIUM CITRATE (PH 6.2), 40% PEG 600.