2WQ6
Structure of the 6-4 photolyase of D. melanogaster in complex with the non-natural N4-methyl T(Dewar)C lesion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.169, 87.486, 91.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.740 - 2.300 |
| R-factor | 0.20265 |
| Rwork | 0.201 |
| R-free | 0.22848 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cvu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.730 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 32351 | |
| <I/σ(I)> | 18.1 | 3.9 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 100 MM HEPES, PH 7, 13-17% PEG1500, 18C |
