2WQ2
GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating iodide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 56.340, 56.340, 56.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.850 - 1.360 |
| R-factor | 0.14359 |
| Rwork | 0.142 |
| R-free | 0.18378 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.876 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.440 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.030 | 0.500 |
| Number of reflections | 6605 | |
| <I/σ(I)> | 16.3 | 2.08 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 2.83 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4 M (NH4)2HPO4, 100 MM TRIS PH 8.5 |
