2WPZ
GCN4 leucine zipper mutant with two VxxNxxx motifs coordinating chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.380, 25.440, 33.700 |
| Unit cell angles | 83.98, 84.86, 78.55 |
Refinement procedure
| Resolution | 19.510 - 1.250 |
| R-factor | 0.18126 |
| Rwork | 0.178 |
| R-free | 0.22339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.330 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 20616 | |
| <I/σ(I)> | 10.8 | 1.85 |
| Completeness [%] | 91.1 | 87.4 |
| Redundancy | 2 | 1.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 4000, 200 MM NA-ACETATE, 0.1 M TRIS, PH 8.5 |
