2WPY
GCN4 leucine zipper mutant with one VxxNxxx motif coordinating chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 33.330, 33.330, 50.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.610 - 1.750 |
| R-factor | 0.21679 |
| Rwork | 0.214 |
| R-free | 0.25239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.603 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.870 |
| Number of reflections | 3548 | |
| <I/σ(I)> | 19.2 | 2.37 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2 M NACL, 100 MM NA-ACETATE, PH 4.6 |
