2WPQ
Salmonella enterica SadA 479-519 fused to GCN4 adaptors (SadAK3, in- register fusion)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.022, 36.967, 178.445 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 34.140 - 1.850 |
| R-factor | 0.22422 |
| Rwork | 0.221 |
| R-free | 0.28831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 29718 | |
| <I/σ(I)> | 10.1 | 1.91 |
| Completeness [%] | 98.3 | 94 |
| Redundancy | 3.97 | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG 3350, 0.2 M KNO3 |
