2WPG
Sucrose Hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.634, 47.658, 87.932 |
| Unit cell angles | 90.00, 98.35, 90.00 |
Refinement procedure
| Resolution | 24.774 - 1.900 |
| R-factor | 0.1564 |
| Rwork | 0.154 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g5a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.012 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.800 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 43650 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 93.9 | 88.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
