2WP7
Crystal structure of deSUMOylase(DUF862)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.033, 42.033, 164.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2234 |
| Rwork | 0.223 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ebq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | |
| Number of reflections | 11515 | |
| <I/σ(I)> | 33.01 | 3.4 |
| Completeness [%] | 92.2 | 80 |
| Redundancy | 7.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 20% (W/V) POLYETHYLENEGLYCOL 3350 AND 200 MM POTASSIUM PHOSPHATE (PH 5.6) |
