2WP3
Crystal structure of the Titin M10-Obscurin like 1 Ig complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 61.674, 61.674, 42.347 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.190 - 1.480 |
| R-factor | 0.17754 |
| Rwork | 0.175 |
| R-free | 0.21741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PREVIOUSLY SOLVED STRUCTURE FROM IN-HOUSE DATA |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.380 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 30142 | |
| <I/σ(I)> | 15.9 | 4.6 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN COMPLEX AT 13 MG/ML 2M AMM SULPHATE, 0.1 M BIS-TRIS PH 5.5 |
