2WOY
Crystal structure of the C-terminal domain of Streptococcus gordonii surface protein SspB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.745, 50.290, 94.370 |
| Unit cell angles | 90.00, 97.44, 90.00 |
Refinement procedure
| Resolution | 46.788 - 1.500 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.20770 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.332 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.320 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 55706 | |
| <I/σ(I)> | 15.9 | 3 |
| Completeness [%] | 98.8 | 92.1 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M CACL2, 0.1 M HEPES PH 7.5, 20 % (W/V) PEG 3350 |
