2WOP
Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-21 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.600, 85.207, 62.472 |
| Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
| Resolution | 62.500 - 1.700 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wol |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.295 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.500 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.131 |
| Number of reflections | 61692 | |
| <I/σ(I)> | 20.5 | 4.8 |
| Completeness [%] | 92.2 | 60 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PH 8 |
