2WOK
Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with bradykinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.511, 83.327, 62.380 |
| Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
| Resolution | 62.140 - 1.700 |
| R-factor | 0.18655 |
| Rwork | 0.185 |
| R-free | 0.22268 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wol |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.464 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.100 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 59885 | |
| <I/σ(I)> | 18 | 2.6 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
