2WOG
Intermediate and final states of human kinesin Eg5 in complex with S-trityl-L-cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-05-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 96.500, 96.500, 124.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.160 - 2.000 |
| R-factor | 0.1579 |
| Rwork | 0.155 |
| R-free | 0.21841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x88 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.548 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0090) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.160 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 87598 | |
| <I/σ(I)> | 12.8 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 22% PEG 3350, 0.15M SODIUM TARTRATE DIBASIC DIHYDRATE, 0.1M MES PH 6.5 |
