2WO5
Structure of wild type E. coli N-acetylneuraminic acid lyase in space group P21 crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.795, 142.167, 84.180 |
| Unit cell angles | 90.00, 109.01, 90.00 |
Refinement procedure
| Resolution | 71.090 - 2.200 |
| R-factor | 0.21424 |
| Rwork | 0.211 |
| R-free | 0.27151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wnn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.462 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0097) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.560 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.470 |
| Number of reflections | 61635 | |
| <I/σ(I)> | 7.6 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS-HCL PH 8.0, 200MM NACL, 18% PEG3350 |
