2WO4
3b' carbohydrate-binding module from the Cel9V glycoside hydrolase from Clostridium thermocellum, in-house data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-05 |
| Detector | RIGAKU RAXIS-IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.897, 73.897, 85.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.980 - 1.850 |
| R-factor | 0.15037 |
| Rwork | 0.148 |
| R-free | 0.19138 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbc |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.978 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.760 |
| Number of reflections | 23006 | |
| <I/σ(I)> | 32.2 | 32.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 1.8 M AMMONIUM SULFATE, 0.1 M CITRIC ACID PH 5.0 |
