2WO1
Crystal Structure of the EphA4 Ligand Binding Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-04 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.736, 73.228, 54.420 |
| Unit cell angles | 90.00, 98.35, 90.00 |
Refinement procedure
| Resolution | 23.750 - 1.850 |
| R-factor | 0.18921 |
| Rwork | 0.187 |
| R-free | 0.23379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nuk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 31875 | |
| <I/σ(I)> | 19.5 | 4 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG 4000, 8% PROPAN-1-OL, AND 100 MM TRIS PH 8.5 |
