2WNU
Complex between c1q globular heads and heparan sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.090, 48.160, 87.730 |
| Unit cell angles | 92.86, 92.20, 113.64 |
Refinement procedure
| Resolution | 19.650 - 2.300 |
| R-factor | 0.20064 |
| Rwork | 0.198 |
| R-free | 0.25558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pk6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.010 | 0.030 |
| Number of reflections | 29046 | |
| <I/σ(I)> | 10.66 | 3.8 |
| Completeness [%] | 91.0 | 88.1 |
| Redundancy | 1.97 | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
