2WN9
Crystal structure of Aplysia ACHBP in complex with 4-0H-DMXBA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-11 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.909, 115.394, 130.607 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.17131 |
Rwork | 0.170 |
R-free | 0.20308 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2byn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.489 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.800 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.050 | 0.390 |
Number of reflections | 132537 | |
<I/σ(I)> | 20.7 | 4.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 26% PEG400, 0.1 M HEPES, PH 7.5, 0.2 M MGCL2 |