2WMY
Crystal structure of the tyrosine phosphatase Wzb from Escherichia coli K30 in complex with sulphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Collection date | 2009-06-05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.902, 53.062, 113.909 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 33.967 - 2.211 |
| R-factor | 0.2091 |
| Rwork | 0.206 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wja |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.884 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 59360 | |
| <I/σ(I)> | 20.1 | 3.2 |
| Completeness [%] | 96.5 | 80.8 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M TRIS-CL PH 8.5, 1.2 M LI2SO4, 0.01M NICL2 |
