2WMP
Structure of the E. coli chaperone PapD in complex with the pilin domain of the PapGII adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.560, 90.470, 64.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 200.000 - 2.300 |
| R-factor | 0.2272 |
| Rwork | 0.227 |
| R-free | 0.28930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pdk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.560 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 17935 | |
| <I/σ(I)> | 4.6 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PROTEIN (COMPLEX) AT 9.9 MG/ML 10-12% PEG 4K 0.1M TRIS-HCL PH 8.5 5% ISOPROPANOL |
