2WMM
Crystal structure of the hinge domain of MukB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 |
| Unit cell lengths | 56.443, 56.443, 115.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2172 |
| Rwork | 0.217 |
| R-free | 0.24010 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.120 |
| Number of reflections | 17535 | |
| <I/σ(I)> | 48.9 | 7.6 |
| Completeness [%] | 96.3 | 89.7 |
| Redundancy | 7.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.1 M D,L-MALIC ACID, 0.1 M HEPES (PH 7.0), 1.4 % (V/V) N-BUTANOL |
