2WML
Crystal Structure of a Mammalian Sialyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-06-26 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.378, 78.010, 98.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.160 - 1.900 |
| R-factor | 0.17503 |
| Rwork | 0.173 |
| R-free | 0.21278 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 2.010 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 26352 | |
| <I/σ(I)> | 16.6 | 3.3 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 4000, 0.1M MES, PH 6.8 |
