2WM8
Crystal structure of human magnesium-dependent phosphatase 1 of the haloacid dehalogenase superfamily (MGC5987)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.340, 62.340, 111.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.450 - 1.750 |
| R-factor | 0.16517 |
| Rwork | 0.163 |
| R-free | 0.19816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.139 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | 0.840 |
| Number of reflections | 37274 | |
| <I/σ(I)> | 15.3 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NA3(CIT),20% PEG 3350 |
