2WLR
Putative thiosulfate sulfurtransferase YnjE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.330, 84.010, 132.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.760 - 1.450 |
| R-factor | 0.1756 |
| Rwork | 0.175 |
| R-free | 0.19599 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.461 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.700 | 1.600 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 74519 | |
| <I/σ(I)> | 19.6 | 3.9 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 11.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 | HANGING DROP, TEMPERATURE 5 C, PEG10000 20%(W/V), HEPES 0.1M PH 7.5 |
