2WLP
Sesbania mosaic virus capsid protein dimer mutant (rCP-DEL-N65-W170K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.880, 41.950, 68.650 |
| Unit cell angles | 81.07, 75.00, 84.25 |
Refinement procedure
| Resolution | 65.650 - 2.650 |
| R-factor | 0.22403 |
| Rwork | 0.221 |
| R-free | 0.29399 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vak |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.445 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.340 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.095 | 0.470 |
| Number of reflections | 10322 | |
| <I/σ(I)> | 9.9 | 2.2 |
| Completeness [%] | 93.2 | 92 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 0.1 M BIS TRIS (PH 6.5) AND 28% PEG 2000 |
