2WLL
POTASSIUM CHANNEL FROM BURKHOLDERIA PSEUDOMALLEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-03 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.840, 105.620, 258.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.955 - 3.650 |
| R-factor | 0.2482 |
| Rwork | 0.246 |
| R-free | 0.28570 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.934 |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.850 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.087 | 0.412 |
| Number of reflections | 14455 | |
| <I/σ(I)> | 4.9 | 1.8 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | PEG-400, MAGNESIUM ACETATE, GLYCINE, HEGA-10, PH 9.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
