2WL8

X-ray crystal structure of Pex19p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH SX-165
Spacegroup name	P 21 21 21
Unit cell lengths	67.150, 91.120, 122.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.450 - 2.050
R-factor	0.20118
Rwork	0.199
R-free	0.23886
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.025
RMSD bond angle	2.093
Data reduction software	MOSFLM
Data scaling software	TRUNCATE
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.470	2.200
High resolution limit [Å]	2.050	2.180
Rmerge	0.060
Number of reflections	45015
<I/σ(I)>	7.57	2.69
Completeness [%]	99.6
Redundancy	6.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2.0M SODIUM MALONATE, PH 5.5