2WKN
gamma lactamase from Delftia acidovorans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.871, 94.621, 152.297 |
| Unit cell angles | 105.71, 90.27, 108.46 |
Refinement procedure
| Resolution | 24.780 - 2.080 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.319 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 176148 | |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 91.2 | 72.1 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 15% PEG 4000 0.1 M SODIUM ACETATE PH 5.6, 50 MM NACL |
