2WKL
Velaglucerase alfa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.371, 285.552, 91.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.700 |
| R-factor | 0.17602 |
| Rwork | 0.173 |
| R-free | 0.23392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.130 | 0.440 |
| Number of reflections | 39866 | |
| <I/σ(I)> | 14.4 | 4.66 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1 M (NH4)2SO4/0.1 M HEPES, PH 7.0, 0.5% (W/V) PEG8000 |
